Table 3.
Adjustments for the ImageJ macro. Background subtraction data and the upper and lower threshold values for determining the stained signal area [%]/image.
| Protein | Tissue | Background Subtraction | Lower Threshold | Upper Threshold |
|---|---|---|---|---|
| Aggrecan | Retina | 50 | 7.55 | 78.88 |
| Optic nerve | 50 | 13.84 | 80.10 | |
| Brevican | Retina | 20 | 5.21 | 35.24 |
| Optic nerve | 20 | 12.18 | 72.30 | |
| Fibronectin | Retina | 50 | 23.45 | 78.31 |
| Optic nerve | 50 | 10.90 | 78.85 | |
| Laminin | Retina | 30 | 11.25 | 75.65 |
| Optic nerve | 30 | 33.98 | 79.83 | |
| Tenascin-C | Retina | 50 | 25.33 | 80.11 |
| Optic nerve | 50 | 18.50 | 73.50 | |
| Tenascin-R | Retina | 20 | 20.00 | 80.00 |
| Optic nerve | 50 | 21.22 | 79.99 | |
| RPTPβ/ζ (473HD) | Retina | 50 | 8.77 | 79.22 |
| Optic nerve | 20 | 18.80 | 75.30 | |
| RPTPβ/ζ (KAF13) | Retina | 50 | 18.01 | 77.02 |
| Optic nerve | 50 | 22.53 | 80.00 |