Table 4.
Primary and secondary antibodies for Western blotting.
| Primary Antibody | Molecular Weight | Dilution | Reference/Source | Secondary Antibody | Dilution | Source |
|---|---|---|---|---|---|---|
| Aggrecan | >100 kDa, >150 kDa |
1:1000 | Millipore | Goat anti-rabbit HRP |
1:10,000 | Jackson ImmunoResearch Labs |
| Brevican | ~50 kDa, >100 kDa |
1:1000 | [46] | Goat anti-guinea pig HRP |
1:5000 | Jackson ImmunoResearch Labs |
| Fibronectin | >250 kDa | 1:10,000 | [47] | Goat anti-rabbit HRP |
1:10,000 | Jackson ImmunoResearch Labs |
| Laminin | 200 kDa, 400 kDa |
1:10,000 | [47] | Goat anti-rabbit HRP |
1:5000 | Jackson ImmunoResearch Labs |
| Tenascin-C (KAF14 antibody) |
~250 kDa, >250 kDa |
1:5000 | [48] | Goat anti-rabbit HRP |
1:10,000 | Jackson ImmunoResearch Labs |
| Tenascin-R (23–14 antibody) |
160 kDa, 180 kDa |
1:1000 | [49] | Goat anti-mouse HRP |
1:5000 | Jackson ImmunoResearch Labs |
| Phosphacan/RPTPβ/ζ (473HD antibody) |
>150 kDa | 1:100 | [50] | Goat anti-rat HRP |
1:5000 | Jackson ImmunoResearch Labs |
| RPTPβ/ζ-isoforms (KAF13 antibody) | >150 kDa | 1:5000 | [50] | Goat anti-rabbit HRP |
1:5000 | Jackson ImmunoResearch Labs |
| α-Tubulin | ~50 kDa | 1:20,000 | Sigma-Aldrich | Goat anti-mouse HRP |
1:10,000 | Jackson ImmunoResearch Labs |