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. 2021 Feb 25;11(3):352. doi: 10.3390/biom11030352

Figure 3.

Figure 3

Effects of tempol on IL-1β-induced inflammatory cytokines gene expression. qRT-PCR performed with IL-1β (B), IL-6 (C) and TNF-α (A) on IL-1β-stimulated CC and treated with tempol (0.5 and 1.0 mM) for 24, 72 and 168 h. GAPDH was used as endogenous controls. CTR, untreated CC; IL-1β, IL-1β-stimulated CC; IL-1β+tempol, IL-1β-stimulated CC and treated with tempol 0.5 and 1.0 mM. Data are representative of at least three independent experiments. One-way ANOVA test. ° compare the LPS-tempol group 72 h to IL1 beta group; *** p < 0.001 vs. CTR 24 h; §§§ p < 0.001 vs. CTR 72 h; +++ p < 0.001 vs. CTR 168 h; ### p < 0.001 vs. LPS 24 h; ##p < 0.01 vs. LPS 24 h; # p < 0.05 vs. LPS 24 h; °°° p < 0.001 vs. LPS 72 h; δδδ p < 0.001 vs. LPS 168 h.