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. 2021 Feb 25;11(3):352. doi: 10.3390/biom11030352

Figure 5.

Figure 5

Effects of tempol on IL-1β-induced MMPs activity and gene expression. IL-1β-stimulated CC were treated with 0.5 and 1.0 mM of tempol for 24, 72 and 168 h. Expression of MMP1 (A), MMP3 (B), MMP9 (C) and MMP13 (D) was evaluated using real time-PCR. CTR, control untreated CC; IL-1β, IL-1β-stimulated CC; IL-1β+tempol, IL-1β-stimulated CC and treated with tempol 0.5 and 1.0 mM. GAPDH was used as endogenous controls. Moreover, MMP activity was evaluated by ELISA kit assay (E,F). Data are representative of at least three independent experiments. One-way ANOVA test. *** p < 0.001 vs. CTR 24 h; §§§ p < 0.001 vs. CTR 72 h; +++ p < 0.001 vs. CTR 168 h; ### p < 0.001 vs. LPS 24 h; ## p < 0.01 vs. LPS 24 h; # p < 0.05 vs. LPS 24 h; °°° p < 0.001 vs. LPS 72 h; δδδ p < 0.001 vs. LPS 168 h.