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. 2021 Feb 25;11(3):180. doi: 10.3390/life11030180

Table 1.

Result of the phytochemical screening of Lepidagathis hyalina Nees stems.

Phytochemicals Type of Test Appearance Results
Alkaloids Mayer’s test Yellow color ++
Wagner test A reddish brown color ++
Carbohydrates Molisch’s test Reddish color ring form ++
Glycosides Shinoda test No deep red color
Reducing sugar Fehling’s test Red precipitate form ++
Benedict’s test Reddish color precipitate form ++
Flavonoids Lead acetate test Florescence yellow color form ++
Saponins Froth test Persistent forth for one hour +
Tannin FeCl3 test Brownish green appears +
Sterols Liebermann–Burchard test No layer form
Triterpene Salkowski test Reddish color form +
Resin FeCl3 test No precipitation
Phenol FeCl3 test Violet color form ++
Quinones HCl test Yellow color present +
Cardiac Glycoside Legal test Brown color +
Coumarins Ammonia test Green color form ++
Cholesterols General test No red rose color
Terpenoids Salkowski’s test Reddish brown not form

Here, ++, highly present; +, moderately present; and −, absent.