Relative expression levels of the genes GS1 (A), GS2 (B), and SRH5 (C), in leaves of sugarcane seedlings inoculated or not (Control) with IAC-BECa-088, IAC-BECa-095, and IAC-BECa-141 = IAC/BECa-141 bacterial strains, and normalized by the expression of GAPDH reference gene transcript levels and with (black bars) or without (gray bars) nitrogen fertilizer, 45 days after transplanting. Different lower-case letters (a,b) indicate significant differences among bacterial treatments within each N fertilization treatment, and capital letters (A, B) indicate significant difference between N fertilization treatments within each bacterial treatment by the Scott-Knott test (p < 0.05).