Table 2.
In vitro activity for the five newly isolated lytic phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, Φ19_OBG) at multiplicity of infection (MOI) 1, and MOI 100 tested against the 24-h-old-preformed Pseudomonas aeruginosa (PA) biofilms.
| Significant PA Biofilm Reduction Numbers (%) | ||||
|---|---|---|---|---|
| 4 h | 24 h | |||
| Phages Tested | MOI 1 | MOI 100 | MOI 1 | MOI 100 a |
| Φ4_ZP1 | 7/18 (38.8) | 9/18 (50.0) | 10/21 (47.6) | 7/21 (33.3) a |
| Φ9_ZP2 | 4/15 (26.6) b | 10/15 (66.6) b | 6/17 (35.2) | 8/17 (47.0) |
| Φ14_OBG | 6/18 (33.3) | 10/19 (52.6) | 10/22 (45.4) | 15/22 (68.1) a,c |
| Φ17_OBG | 14/25 (56.0) | 12/26 (46.1) | 10/31 (32.2) | 10/31 (32.2) c |
| Φ19_OBG | 11/24 (45.8) | 14/24 (58.3) | 6/24 (25.0) | 10/24 (41.6) |
| Total | 42/100 (42.0) | 55/102 (53.9) | 42/115 (36.5) | 50/115 (43.4) |
PA biofilms from PAO1, and 33 cystic fibrosis (CF) Pa_Ph isolates after 4 and 24 h exposures. Numbers and percentages (%) of statistically significant PA biofilm reductions in crystal-violet stained samples. Statistically significant difference by χ2 test: a Φ4_ZP1 vs. Φ14_OBG p < 0.05; b Φ9_ZP2: MOI 1 vs. MOI 100 p < 0.001; c Φ14_OBG vs. Φ17_OBG p < 0.01.