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. 2021 Feb 25;10(3):346. doi: 10.3390/antiox10030346

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Srx deletion on L-Prx V maturation in adrenal gland. Whole lysates (A), cytosolic (B), and mitochondrial (C) fractions obtained after subcellular fractionation of adrenal gland homogenates from wild type and Srx KO mice were subjected to immunoblot analysis with antibodies to indicated proteins. Two different blot exposures are shown for Prx V blot in (C). The band intensities of S-Prx V in whole lysates and cytosol are normalized to the intensity of GAPDH (D), and the band intensities of S-Prx V and I-Prx V in mitochondria are normalized to the intensity of VDAC (E,F). Data are means ± SE (n = 3 per group),* p < 0.05.