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. 2021 Mar 25;9(3):e001965. doi: 10.1136/jitc-2020-001965

Figure 4.

Figure 4

CD73 protein expression in HGSC is associated with worse prognosis and promotes epithelial-to-mesenchymal transition (EMT). (A) Disease-free survival analysis of CD73 protein expression in epithelial (cytokeratin+) areas of HGSC tumors (n=720 patient). (B) Gene ontology analysis of CD73-High immunoregulatory (IMR) and differentiated (DIF) HGSC. (C) Gene-silencing of ZEB1 was performed in human SKOV-3 ovarian tumor cells and CD73 surface expression measured by flow cytometry. (D) Gene-silencing of CD73 was performed in SKOV-3 cells and cytokeratin (CK)−19, vimentin and N-cadherin protein expression measured by Western blot (means±SEs) of three experiments are shown. (E) Disease-free survival analysis of CD73 protein expression in αSMA+ stromal areas of HGSC tumors (n=704 patients). (F) Disease-free survival analysis according to stromal (αSMA+) CD73 expression and intratumoral CD8 expression (n=585 patients). HGSC, high-grade serous ovarian cancer.