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. 2021 Mar 25;9(3):e002035. doi: 10.1136/jitc-2020-002035

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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bbT485 TCR-Ts expressing an allo-derived TCR exhibits superior epitope binding characteristics compared with bbT476 expressing the auto-derived TCR. (A, B) Dot plots indicating the percentage of HLA-A2-GVYDGREHTV pentamer-positive CD8⁺ T cells and bar graphs showing median fluorescence intensity (MFI) within respective gates. (A) Analysis of the original T-cell clones. (B) Analysis of healthy donor-derived PBL transduced and enriched to express either the auto-derived or the allo-derived TCR. (C) Dose–response curves determining the EC₅₀ value for bbT476 or bbT485 CD8⁺ T cells. Shown are the relative individual values and the non-linear regression curve of IFN-γ determined by ELISA 16 hours after co-culture with T2 cells pulsed with different concentrations of the GVY peptide. (B, C) Data shown are representative of 3 different donors for each tested TCR.