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. 2021 Mar 25;9(3):e002035. doi: 10.1136/jitc-2020-002035

Figure 5.

Figure 5

Co-receptor independence of the allo-derived TCR enables CD4+ T-cell-mediated anti-tumor response. Graphs showing different read-outs of activation of CD8+ and CD4+ T cells expressing transgenic TCRs (bbT476 or bbT485) or not (UTD, untransduced) in co-culture with MAGE-A4-positive HLA-A*02:01-positive MelA375 cells. (A) Bar graphs displaying IFN-γ concentrations in supernatants (left panel) and Granzyme B (GzmB) expression among CD8+ and CD4+ T cells determined by flow cytometry (right panel) 16 hours after co-culture. (B) Graph showing the cell number of MelA375 cells expressing NucLight Red live-cell labeling reagent (mKate) for real-time imaging in co-culture with T cells. No effectors=without T cells. (C) Quantification of cytokine levels in supernatants (left panel) and CD154 expression among CD4+ T cells determined by flow cytometry (right panel) 16 hours and 6 hours after co-culture, respectively. Data shown are representative of 3 different donors for each tested TCR.