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. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: ACS Nano. 2020 Jul 22;14(8):9243–9262. doi: 10.1021/acsnano.0c04707

Figure 4.

Figure 4.

Nonviral delivery systems for in vivo genome editing via intracranial injection. (A) Schematic representation of the formation of CRISPR-Cas9 nanocomplex delivery system. Local administration of CRISPR-Cas9 nanocomplexes successfully edited target genes in post-mitotic neurons of the adult brain. Reproduced with permission from ref 75. Copyright 2019 Springer Nature. (B) Schematic representation of CRISPR-Gold synthesis. When injecting mGluR5–CRISPR into the striatum of wild-type (WT) and Fmr1 knockout (Fmr1 KO) mice, Grm5 mRNA levels were reduced by 40–50%. Reproduced with permission from ref 76. Copyright 2018 Springer Nature. Single-guide RNA, sgRNA; ribonucleoprotein, RNP.