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. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: ACS Nano. 2020 Jul 22;14(8):9243–9262. doi: 10.1021/acsnano.0c04707

Figure 6.

Figure 6.

Nonviral delivery systems for in vivo genome editing via intra-inner ear injection. (A) Cationic lipids mediated delivery of proteins into mammalian cells through engineering proteins by fusion or noncovalent complexation with polyanionic macromolecules. Local injection of Cas9/sgRNA complexes delivered by RNAiMAX or Lipofectamine 2000 into the scala media successfully resulted in loss of green fluorescent protein (GFP) expression in hair cells. Reproduced with permission from ref 81. Copyright 2015 Springer Nature. (B) Scala media injection of Cas9/Tmc1-mut3 complexed with Lipofectamine 2000 (Cas9–Tmc1-mut3–lipid) promoted hair cell survival and reduced hearing loss in Tmc1Bth/+ mice. Reproduced with permission from ref 82. Copyright 2018 Springer Nature.