Table 4.
Primers used for the RT-qPCR.
| Gene | Primer | Primer Sequence 5’–3’ | Product Size (bp) |
|---|---|---|---|
| IL-6 | Forward Reverse | CAGGTCTATTTTGGGATCATTGCC TCCCTGATTTCTAAGTGTTGCTGT |
189 |
| TNF-α | Forward Reverse | CACCTCAGACAAAATGCTCTTCAC CTCACACATCTCCTTTCTCATTGC |
100 |
| ZO-1 | Forward Reverse | TACCTCTTGAGCCTTGAACTT CGTGCTGATGTGCCATAATA |
248 |
| Occludin | Forward Reverse | GCCCAGGCTTCTGGATCTATGT GGGGATCAACCACACAGTAGTGA |
124 |
| Claudin-1 | Forward Reverse | GCTGGGTTTCATCCTGGCTTCT CCTGAGCGGTCACGATGTTGTC |
110 |
| β-actin | Forward Reverse | AGTGTGACGTTGACATCCGT GCAGCTCAGTAACAGTCCGC |
298 |