Table 1.
Microbiological, biochemical, and molecular identification of isolates. Escherichia coli and closely related isolates from selected animal species as identified using an API 20E Gram-negative Microbial Identification Kit, and molecular identification methods (PCR and Sequencing) using 16E1/16E2/16E3 and P3mod/P5 Primers.
| Animal Species | Number of Samples Processed | Total Isolates (% Yield) |
Identified Bacteria by Species | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Biochemical Identification Using API® 20E (n = 66) |
Molecular Detection Using 16E1/16E2/16E3 and P3mod/P5 Primers (n = 65) |
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|
Escherichia coli Group 1 n (%) |
Burkholderia cepacian (%) |
Kluyvera spp. n (%) |
Unidentified n (%) |
Escherichia coli spp. n (%) |
Escherichia fergusoniin (%) |
Shigella spp. n (%) |
Enterobacteriaceae bacteriumn (%) |
||||
| Impala | 16 | 15 (93.8) | 6 (40) | 0 | 1 (6.7) | 8 (53.3) | 11 (78.6) | 0 | 3 (21.4) | 0 | |
| Buffalo | 13 | 8 (61.5) | 5 (62.5) | 0 | 0 | 3 (37.5) | 6 (75) | 1 (12.5) | 1 (12.5) | 0 | |
| Ostrich | 7 | 6 (85.7) | 5 (83.3) | 0 | 0 | 1 (16.7) | 5 (83.3) | 0 | 1 (16.7) | 0 | |
| Cattle | 20 | 16 (80) | 11 (68.8) | 0 | 0 | 5 (31.3) | 13 (81.2) | 0 | 3 (18.8) | 0 | |
| Pig | 18 | 15 (83.3) | 11 (73.3) | 0 | 0 | 4 (26.7) | 10 (66.7) | 0 | 4 (26.7) | 1 (6.7) | |
| Goat | 10 | 6 (60) | 1 (16.7) | 1 (16.7) | 0 | 4 (66.7) | 3 (50) | 0 | 3 (50) | 0 | |
| Total | 84 | 66 (78.6) | 39 (59.1) | 1 (1.5) | 1 (1.5) | 25 (37.9) | 48 (72.7) | 1 (1.5) | 15 (22.7) | 1 (1.5) | |
|
Sequence percentage homology
(%) |
Minimum | 90.00 | 1st Quantile | 98.10 | |||||||
| Mean | 98.29 | Median | 99.00 | ||||||||
| Maximum | 100.00 | 3rd Quantile | 99.30 | ||||||||