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. 2021 Feb 26;7(3):169. doi: 10.3390/jof7030169

Figure 1.

Figure 1

In silico PCR showing homology regions with sequences deposited in the GenBank. (a) Primers were designed to match the target gene gp43 exon-2. Primers PbraCx-F and PbraCx-R targeting P. brasiliensis complex DNA produced an amplicon with an expected size of 308 bp, while primers Plu-F and Plu-R targeting P. lutzii DNA generated an amplicon with 142 bp. (b) Interspecific polymorphisms in the primers’ annealing region between P. brasiliensis complex isolates and P. lutzii or between Paracoccidioides and the closely related species Lacazia loboi supported the specificity of the primers developed.