Skip to main content
. 2021 Feb 26;7(3):169. doi: 10.3390/jof7030169

Figure 6.

Figure 6

Detection of Paracoccidioides DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. (a) Agarose gel electrophoresis showing successful detection of Paracoccidioides DNA using a duplex PCR assay. Remarkably, in four cases (i.e., FFPE04, FFPE06, FFPE10, and FFPE11), we confirmed co-infection due to P. brasiliensis s.l. and P. lutzii. Controls include DNA from A549 cells; Pb18: P. brasiliensis complex positive control; Pb01: P. lutzii positive control; NTC: no template control. (b) Median-joining haplotype network of Paracoccidioides species, covering all the 308-bp FFPE-derived sequences found in this study. The circumference diameter is proportional to the frequency of the haplotype. White dots represent mutational steps, and asterisks correspond to co-infections. Unsampled or extinct haplotypes in the population are indicated as black dots (median vectors). FFPE-derived sequences were clustered in two haplotypes (H5 or H6). Further information about sequence sources and GenBank accession numbers can be found in Supplementary Table S2.