Figure 5.

Glutamine metabolism through glutaminolysis and the TCA cycle is enhanced in CRPC. Glutamine metabolism was assessed in LNCaP and PC-3 cells based on (A) consumption rate of [U-¹³C]glutamine, (B) the fractional enrichment of aspartate and glutamate of cells labeled with [U-¹³C]glutamine, and (C) enzymatic activity of GLS. Flux through glutaminolysis and the TCA cycle was determined in ADPC and CRPC TRAMP tumors based on (D) the fractional enrichment of aspartate and glutamate of tumors labeled with [U-¹³C]glutamine and (E) enzymatic activity of GLS. (* p < 0.05, ** p < 0.01).