Figure 7.

Glutathione redox capacity is upregulated in CRPC. Glutathione redox capacity was assessed in LNCaP and PC-3 cells based on (A) fractional enrichment of glutathione in cells labeled with [U-¹³C]glutamine, (B) ratio of reduced glutathione (GSH) relative to total glutathione (GSH + GSSG), and (C) intracellular NADP⁺/NADPH. Glutathione redox balance was assessed in ADPC and CRPC TRAMP tumors based on (D) fractional enrichment of glutathione in tumors labeled with [U-¹³C]glutamine and (E) total glutathione (* p < 0.05, ** p < 0.01, **** p < 0.0001).