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. 2021 Feb 26;13(3):308. doi: 10.3390/pharmaceutics13030308

Figure 4.

Figure 4

Corneal stroma structures and keratocytes. (A) Reproduced with permission form [42], Elsevier, 2018. (a) Images of extrusion bio-printed corneal stroma. Scale bar 20 mm. (b) Representative live/dead stain images using fluorescence microscopy after 3D bioprinting, showing live (green) and dead (red) cells. Scale bar 250 µm. (B) Reproduced with permission from [44], IOP Science, 2019. (a) Stereomicrographs of the methacrylate gelatin scaffold. Scale bar 1 mm. (b) Live/dead cell viability assay of 3D printed GelMA scaffolds on day 21, showing live (green) and dead (red) cells. Scale bar 100 μm. (C) Reproduced with permission from [37], Wiley, 2019. (a) Macrograph of ink-jet bio-printed 3D type I collagen/agarose scaffold. (b) Human corneal stroma keratocytes (CSK) stained with live/dead staining 7 days after bioprinting, showing live (green) and dead (red) cells. It is noticeable that the CSK is spreading and shows typical dendritic shapes in the scaffold. Scale bar 100 µm.