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. 2021 Feb 26;9(3):494. doi: 10.3390/microorganisms9030494

Figure 1.

Figure 1

Screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) open reading frames (ORFs) that regulate caspase-1-mediated IL-1β production. Human embryonic kidney 293T (HEK 293T) cells were co-transfected with vectors expressing caspase-1 and pro-IL-1β, plus either pEF1α-DEST or pEF1α-DEST expressing complementary DNAs (cDNAs) encoding 28 SARS-CoV-2 ORFs. After 24 h, mature IL-1β in culture supernatants was measured by enzyme-linked immunosorbent assay (ELISA). * p < 0.001 by unpaired two-tailed Student’s t-tests. Data shown are representative of three independent experiments.