Fig 4. The γ₁34.5 protein interacts with RIG-I.

(A and B) The γ₁34.5 protein interacts with endogenous RIG-I in infected cells. MEFs were infected with wild-type HSV-1 or Δγ₁34.5 (10 pfu/cell). At 8 h postinfection, the cells were processed for immunoprecipitation (IP) with anti-RIG-I antibody or normal mouse IgG (A), or with anti-γ₁34.5 antibody or normal rabbit IgG (B). Whole-cell lysates (WCL) and precipitated proteins were probed with antibodies against RIG-I, γ₁34.5, ICP27 and β-actin. (C and D) HSV-1 γ₁34.5 interacts with RIG-I in the absence of other viral proteins. HEK-293T cells were transfected with Myc-RIG-I together empty vector (Vec), Flag-γ₁34.5 or Flag-mCherry for 36 h. Cell lysates were subjected to immunoprecipitation (IP) with anti-Myc antibody (C) or anti-Flag antibody (D). Precipitated proteins and WCLs were probed with antibodies against Flag, Myc, and β-actin. The data are representative of results from three independent experiments.