Table 1.
Summary of evaluated LOF and GOF mutations in the SARS-CoV-2 genome (N = 15 × 2).
| SARS-CoV-2 Target | Loss of Function | Gain of Function |
|---|---|---|
| aiPSC-WT | N/A | N/A |
| aiLUNG (i.e. Uninfected) | N/A | N/A |
| aiLUNG + SARS-CoV-2 | N/A | N/A |
| Spike-RBD Mutation | −1, Locked OFF | +1, Locked ON |
| Furin Mutation | −1, Locked OFF | +1, Locked ON |
| NSP12 Mutation (RdRP) | −1, Locked OFF | +1, Locked ON |
| orf1ab Mutation | −1, Locked OFF | +1, Locked ON |
| orf10 Mutation | −1, Locked OFF | +1, Locked ON |
| (N)ucleoprotein Mutation | −1, Locked OFF | +1, Locked ON |
| (M)embrane Mutation | −1, Locked OFF | +1, Locked ON |
| NSP3 Mutation | −1, Locked OFF | +1, Locked ON |
| orf7a Mutation | −1, Locked OFF | +1, Locked ON |
| orf8 Mutation | −1, Locked OFF | +1, Locked ON |
| NSP5 Mutation | −1, Locked OFF | +1, Locked ON |
| (S)pike Mutation | −1, Locked OFF | +1, Locked ON |
| (E)nvelope Mutation | −1, Locked OFF | +1, Locked ON |
| NSP13 Mutation (Helicase) | −1, Locked OFF | +1, Locked ON |
| orf3a Mutation | −1, Locked OFF | +1, Locked ON |
NSP = Non-Structural Protein, RdRP = RNA-dependent RNA polymerase, orf = open reading frame, aiLUNG = Wild Type (uninfected), aiLUNG + SARS-CoV-2 = aiLUNG-COVID-19.