. 2021 Feb 28;10(3):241. doi: 10.3390/antibiotics10030241

Table 2.

The PCR primers’ specification and the PCR parameters applied in the present study.

Gene Detected	PCR Primer Name	Manufacturer	Primer Sequence 5′→3′	Tm (°C)	Annealing Temperature (°C)	Product Size (bp)
*phzS*	phzS F	Sigma	TCGCCATGACCGATACGCTC	55.9	63	1752
*phzS*	phzS R		ACAACCTGAGCCAGCCTTCC	55.9	63	1752
*exoT*	exoT F		AATCGCCGTCCAACTGCATGCG	58.6	64	152
*exoT*	exoT R		TGTTCGCCGAGGTACTGCTC	55.9	64	152
*exoY*	exoY F		CGGATTCTATGGCAGGGAGG	55.9	64	289
*exoY*	exoY R		GCCCTTGATGCACTCGACCA	55.9	64	289
*exoU*	exoU F		CCGTTGTGGTGCCGTTGAAG	55.9	64	134
*exoU*	exoU R		CCAGATGTTCACCGACTCGC	55.9	64	134
*toxA*	toxA F	Integrated DNA Technologies	GGTAACCAGCTCAGCCACAT	57.4	52	352
*toxA*	toxA R		TGATGTCCAGGTCATGCTTC	54.8	52	352
*algD*	algD F		ATGCGAATCAGCATCTTTGGT	55.2	50	1310
*algD*	algD R		CTACCAGCAGATGCCCTCGGC	62.5	50	1310
*exoS*	exoS F		CTTGAAGGGACTCGACAAGG	55.2	53	504
*exoS*	exoS R		TTCAGGTCCGCGTAGTGAAT	56.2	53	504
*phzM*	phzM F	Genomed	ATGGAGAGCGGGATCGACAG	55.9	54	875
*phzM*	phzM R		ATGCGGGTTTCCATCGGCAG	55.9	54	875
*pilA*	pilA F		ACAGCATCCAACTGAGCG	50.3	59	1675
*pilA*	pilA R		TTGACTTCCTCCAGGCTG	50.3	59	1675
*pilB*	pilB F		TCGAACTGATGATCGTGG	48.0	56	408
*pilB*	pilB R		CTTTCGGAGTGAACATCG	48.0	56	408