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. 2021 Feb 27;7(3):172. doi: 10.3390/jof7030172

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), translation elongation factor 1-α (EF-1α) and one SCAR marker (OPA 10-2) multilocus phylogenetic tree developed using the Maximum Likelihood Method, based on the Tamura–Nei model. The tree with the greatest log likelihood (-3746.14) is shown. Relationships between the 42 isolates from pomegranate sourced in southern Italy and the CBS reference isolates of Alternaria alternata, A. arborescens (in bold CBS isolates used as reference for diverse morphotypes) and other Alternaria spp. The six morphotypes are highlighted in different colors.