Figure 2.

VA1 replication is delayed in comparison to HAstV-1. Caco-2 cells were mock-infected or infected with HAstV-1 or VA1 (MOI of 2). At various time points, cells were stained for viral capsid and double-stranded RNA (dsRNA) by immunofluorescent staining. (A) Representative images, taken at 40× magnification on EVOS FL, of HAstV-1 and VA1 capsid production and dsRNA formation at 24 hpi. The percent of dsRNA positive (B) and capsid positive (C) cells at the indicated timepoints was quantitated.