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. 2021 Mar 26;7(13):eabc6345. doi: 10.1126/sciadv.abc6345

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Fig. 6 — (A) Light microscopy images of Vps4B knockdown HeLa cells grown on glass (i) before and (ii to v) at various time points after damage—(left) bright-field and (right) CHMP4B-EGFP imaging. EGFP fluorescence is shown using inverted grayscale. The damage area is 3 μm in diameter. (B) Cryo-ET of damage sites of Vps4B knockdown cells showing actin-filled plasma membrane protrusions, pearling/budding profiles, shed vesicles, protein densities observed at certain sites of high membrane curvature in budding profiles and shed vesicles, chains of budding profiles, shed membrane protrusions devoid of F-actin, and nested protrusions. Samples sizes for quantifications: 11 tomograms for Vps4B knockdown (versus 10 tomograms for wild type). Scale bars, 10 μm (A) and 200 nm (B).