Table 1.
Strengths and limitations of various methods to diagnose SARS-CoV-2 (evaluated for each sample).
| Method | Turnaround time | Equipment requirement | Reagent requirement | Diagnosis value |
Refs. | ||
|---|---|---|---|---|---|---|---|
| Sensitivity/specificity | Capacity to diagnose asymptomatic infection | Potential for mutation detection | |||||
| RT-qPCR | 2 h | Medium | High | High/High | High | Medium | [27,28,30,35,80] |
| RT-LAMP, RT-RPA, etc. |
30-45 min | Low | High | High/High | High | Medium | [[39], [40], [41], [42]] |
| Sequencing | 8 h | High | Low | Low/High | Low | High | [1,44,46] |
| CRISPR based strategy | 20-50 min | Low | High | High/High | High | Medium | [[48], [49], [50], [51],81] |
| Antibody detection | Huge diversity (some <1 h) | Low | Medium | Medium/Medium (due to immunodeficiency and cross-activity) | Medium | Low | [1,[57], [58], [59], [60], [61], [62], [63], [64], [65], [66],68,69,71,77] |