Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Rattus norvegicus) | GRIK2_RAT | Provided by Dr. Janet Fisher (University of South Carolina) | P42260 | |
| Gene (Rattus norvegicus) | GRIK5_RAT | Provided by Dr. Janet Fisher (University of South Carolina) | Q63273 | |
| Cell line (Homo sapiens) | HEK293S GnTI- | ATCC | ATCC, Cat. No. CRL-3022 | |
| Cell line (Homo sapiens) | HEK293T/17 | ATCC | ATCC, Cat. No. CRL-11268 | |
| Cell line (insect) | Sf9 | Expression Systems, courtesy of Dr. Xin-Yun Huang (Weill Cornell Medical College) | Expression Systems, Cat. No. 94–001S | |
| Recombinant DNA reagent | pEZTBM vector | doi: 10.1016/j.str.2016.03.004 | https://www.addgene.org/74099/ | |
| Chemical compound, drug | 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) | Tocris | Tocris, Cat. No. 1045 | |
| Chemical compound, drug | L-Glutamate | Sigma | Sigma, Cat. No. G1251 | |
| Software, algorithm | pClamp 9, Clampfit 10.5 | Molecular Devices, LLC | RRID:SCR_011323 | http://www.moleculardevices.com/products/software/pclamp.html |
| Software, algorithm | OriginPro 2020 | OriginLab | RRID:SCR_014212 | https://www.originlab.com/2020 |
| Software, algorithm | Relion 3.1 | doi:10.7554/eLife.42166 | RRID:SCR_016274 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| Software, algorithm | cryoSPARC 2 | doi:10.1038/nmeth.4169. | RRID:SCR_016501 | https://cryosparc.com/ |
| Software, algorithm | CTFFIND 4.1 | doi:10.1016/j.jsb.2015.08.008 | RRID:SCR_016732 | http://grigoriefflab.janelia.org/ctffind4 |
| Software, algorithm | UCSF Chimera | doi:10.1002/jcc.20084 | RRID:SCR_004097 | http://plato.cgl.ucsf.edu/chimera/ |
| Software, algorithm | UCSF ChimeraX | doi:10.1002/pro.3943 | RRID:SCR_015872 | https://www.cgl.ucsf.edu/chimerax/ |
| Software, algorithm | Bsoft | doi:10.1006/jsbi.2001.4339 | RRID:SCR_016503 | https://lsbr.niams.nih.gov/bsoft/ |
| Software, algorithm | HOLE | doi:10.1016/s0263-7855(97)00009-x | http://www.holeprogram.org | |
| Software, algorithm | COOT 0.9 | doi:10.1107/S0907444910007493 | RRID:SCR_014222 | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Software, algorithm | Phenix 1.14 | doi:10.1107/S2059798319011471 | RRID:SCR_014224 | https://www.phenix-online.org/ |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | http://www.graphpad.com/ |
| Software, algorithm | MolProbity | doi:10.1107/S0907444909042073 | RRID:SCR_014226 | http://molprobity.biochem.duke.edu |