Table 2. Cryo-electron microscopy (cryo-EM) data collection and processing.
| GluK2/K5-apo | GluK2/K5-CNQX (full-length) |
GluK2/K5-CNQX (ATD) |
GluK2/K5-CNQX (LBD-TMD) |
GluK2/K5-L-Glu (full-length) |
GluK2/K5-L-Glu (ATD) |
GluK2/K5-L-Glu (LBD-TMD) |
|
|---|---|---|---|---|---|---|---|
| Magnification | 81,000 | 36,000 | 36,000 | 36,000 | 36,000 | 36,000 | 36,000 |
| Voltage (kV) | 300 | 200 | 200 | 200 | 200 | 200 | 200 |
| Electron exposure (e–/Å2) | 51.23 | 50–53 | 50–53 | 50–53 | 50–53 | 50–53 | 50–53 |
| Defocus (μm) | 1.6 | 0.4–4.8 | 0.4–4.8 | 0.4–4.8 | 0.4–4.8 | 0.4–4.8 | 0.4–4.8 |
| Pixel size (Å) | 1.083 | 1.096 | 1.096 | 1.096 | 1.096 | 1.096 | 1.096 |
| Symmetry imposed | C1 | C1 | C1 | C2 | C1 | C1 | C2 |
| Initial particle images (#) | 1,778,627 | 51,898,826 | 51,898,826 | 51,898,826 | 28,586,529 | 28,586,529 | 28,586,529 |
| Final particle images (#) | 90,027 | 1,021,916 | 540,580 | 184,945 | 573,403 | 241,849 | 140,028 |
| Map resolution (Å) | 7.5 | 5.3 | 3.6 | 4.2 | 5.8 | 3.8 | 4.3 |
| Fourier shell correlation (FSC) threshold | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 6.5–8.0 | 4.0–6.0 | 3.5–4.0 | 4.0–5.0 | 4.0–6.0 | 3.5–4.0 | 4.0–5.0 |