Fig. 1.

Characterization of SARS-CoV-2 Nucleoprotein interactome. A) Vero E6 cells were infected with the SARS-CoV-2 isolate (2019-nCoV/Italy-INMI1) for 1 h at 37 °C at a multiplicity of infection (MOI) 0.01. At 24 h post infection, cells were harvested and protein extracts were subjected to immunoprecipitation using an anti-SARS-CoV Nucleoprotein antibody. Immunopurified complexes were analyzed by immunoblotting. NI: non-infected; N PROTEIN: Nucleoprotein. B) Network of Nucleoprotein interacting proteins generated with STRING, with continuous lines to represent direct interactions (physical), while dotted lines to represent indirect ones (functional). Line thickness indicates the strength of data support between edges. C) Top five biological processes enriched in Nucleoprotein interacting proteins based on gene ontology terms, generated with STRING.