FIG. 2.
Myogenic properties of newly established cell lines from WT and dystrophic dogs. (a) Immunocytochemistry showing MyHC expression on day 6 and dystrophin on day 8 after triggering differentiation in WT clone 2 and CXMDJ clone 9 (green), respectively. Nuclei were visualized with DAPI (blue). Scale bar: 50 μm. NC, negative control without primary antibody. (b) Expression of muscle regulatory factors, including MYOD1, MYOG, NCAM1, and DES, evaluated by RT-PCR of immortalized WT clone 2 and CXMDJ clone 9. N = 3. DES, desmin; MyHC, myosin heavy chain; MYOD1, myogenic differentiation 1; MYOG, myogenin; NCAM1, neural cell adhesion molecule 1; RT-PCR, reverse transcription polymerase chain reaction.