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. 2021 Mar 18;2021:6687089. doi: 10.1155/2021/6687089

Figure 2.

Figure 2

Effects of Saussureae Radix (SR) on (a, b) the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase- (COX-) 2, (c) the mRNA expression of heme oxygenase- (HO-) 1, and (d) the protein expression of HO-1 and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf-2). Control cells were incubated with only fresh DMEM. BV2 cells were stimulated with lipopolysaccharide (LPS) for 12 (iNOS and COX-2), 6 (HO-1 protein), or 3 h (HO-1 mRNA and Nrf-2). (b, d) The histogram graphs show protein expression levels relative to those of the housekeeping protein. (d) The cultured BV2 microglia were incubated with anti-Nrf-2 (green) and DAPI (blue). Fluorescence was developed using the Alexa Fluor 488-conjugated anti-rabbit secondary antibody. #p < 0.05 (vs. control); ∗p < 0.05 and †p < 0.001 (vs. LPS).