Table 1.
Summary of experimental results on genes identified to be important for surface-associated motility in A. baumannii ATCC 17978
| Locus tag in ATCC 17978 | Annotation/gene name | Predicted function | Motility deficiencya | Growth deficiencyb | Attenuation in Galleria mellonella infectionc | Pellicle biofilm formationd | MIC valuese | ||
|---|---|---|---|---|---|---|---|---|---|
| Ampicillin | Imipenem | Tetracycline | |||||||
| Purine/pyrimidine/folate biosynthesis | |||||||||
| A1S_2187 | purH | Phosphoribosylaminoimidazolecarboxamide formyltransferase (purine synthesis) | + | Y | N.s. | ↑** | N.s. | N.s. | N.s. |
| A1S_2251 | purF | Amidophosphoribosyltransferase (purine synthesis) | ++ | Y | N.s. | N.s. | R*** | N.s. | R* |
| A1S_2605 | purM | Phosphoribosylaminoimidazole synthetase (purine synthesis) | +++ | Y | * | ↑* | R*** | N.s. | S* |
| A1S_2964 | purE | Phosphoribosylaminoimidazole carboxylase mutase subunit (purine synthesis) | +++ | Y | N.s. | ↑*** | R*** | S** | S* |
| A1S_1566 | 6-Pyruvoyl-tetrahydropterin synthase (folate biosynthesis) | +++ | Y | ** | ↓**** | R**** | N.s. | N.s. | |
| A1S_2687 | carB | Carbamoylphosphate synthase subunit (pyrimidine synthesis) | ++ | Y | **** | ↓**** | N.s. | S*** | S** |
| Alarmones/ stress metabolism | |||||||||
| A1S_0414 | Ap5A pyrophosphatase | ++ | Y | * | ↓**** | R**** | N.s. | N.s. | |
| A1S_1624 | Ap4A hydrolase | ++ | Y | N.s. | N.s. | N.s. | S*** | N.s. | |
| RNA modification/ regulation | |||||||||
| A1S_0447 | rpmG | 50S ribosomal protein L33 | +++ | Y | N.s. | N.s. | N.s. | S* | S** |
| A1S_0778 | metG | Methionyl-tRNA synthetase | +++ | Y | **** | ↓**** | N.s. | N.s. | S*** |
| A1S_2182 | gidA | Glucose-inhibited division protein A, FAD-binding protein | ++ | Y | N.s. | ↓**** | R* | N.s. | N.s. |
| Oxidative stress | |||||||||
| A1S_0530 | Rhodanese domain-containing protein, sulfurtransferase | ++ | N | N.s. | ↑**** | N.s. | N.s. | N.s. | |
| A1S_3366 | gshA | Gamma-glutamate-cysteine ligase | ++ | Y | * | N.s. | R*** | N.s. | N.s. |
| Outer membrane proteins | |||||||||
| A1S_1970 | Outer membrane protein (Omp85 family) | ++ | Y | ** | ↓** | R**** | S* | N.s. | |
| A1S_2840 | ompA | Outer membrane protein | ++ | N | **** | ↓**** | N.s. | R*** | S* |
| A1S_3297 | Putative outer membrane protein | + | Y | ** | ↑** | R*** | R* | N.s. | |
| 1,3-diaminopropane biosynthesis | |||||||||
| A1S_2453 | ddc | l-2,4-Diaminobutyrate decarboxylase, biosynthesis of 1,3-diaminopropane (DAP) | +++ | Y | N.s. | ↓**** | N.s. | N.s. | S*** |
| A1S_2454 | dat | l-2,4-Diaminobutyrate:2-ketoglutarate 4-aminotransferase, biosynthesis of 1,3-diaminopropane (DAP) | +++ | Y | * | ↓**** | N.s. | N.s. | S*** |
| Lipopeptide synthesis/ export | |||||||||
| A1S_0113 | Acyl-CoA dehydrogenase (putative lipoprotein biosynthesis) | ++ | N | N.s. | ↓** | R* | N.s. | N.s. | |
| A1S_0116 | RND superfamily transporter (efflux pump) | ++ | N | N.s | ↓** | R*** | N.s. | N.s. | |
| DNA modification/repair/uptake | |||||||||
| A1S_0222 | aamA | Adenine-specific methyltransferase | ++ | Y | *** | ↓**** | N.s. | S*** | S** |
| A1S_2334 | sahH | S-Adenosyl-l-homocysteine hydrolase | +++ | Y | * | N.s. | R*** | N.s. | N.s. |
| A1S_2587 | ruvA | Holliday junction helicase subunit A | ++ | Y | N.s. | ↓**** | R*** | N.s. | S** |
| A1S_2610 | comEC | Competence factor | ++ | N | ** | N.s. | N.s. | N.s. | N.s. |
| Other | |||||||||
| A1S_0065 | galE | UDP-glucose 4-epimerase, lipopolysaccharide biosynthesis | ++ | N | **** | ↑**** | R*** | N.s. | R* |
| A1S_0806 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase | +++ | Y | N.s. | ↑**** | R*** | R**** | N.s. | |
| A1S_1055 | Soluble lytic murein transglycosylase | ++ | N | ** | ↑** | N.s. | N.s. | S* | |
| A1S_2761 | prpF | 2-Methylaconitate isomerase | +++ | Y | N.s. | ↓**** | R* | N.s. | S** |
| A1S_3026 | Hyp. secreted ribonuclease T2 (predicted secretion signal) | ++ | N | N.s | N.s. | R**** | R*** | N.s. | |
| A1S_3129 | astB | Succinylarginine dihydrolase | +++ | Y | N.s. | ↓**** | R*** | N.s. | S** |
aCompared to ATCC 17978 wild type strain (WT); diameter of the spreading zone was measured: ‘+++, 0–4 mm; ‘++ < 4–9 mm; ‘+, <9 mm
bComparison of bacterial growth curves. Y yes growth deficiency compared to WT was observed, N no growth deficiency was observed
cCompared to ATCC 17978 WT; unpaired t test was performed after 5 days p.i.: N.s. not significant, *P value ≤ 0.05; **P value ≤ 0.01; ***P value ≤ 0.001; ****P value ≤ 0.0001
dCompared to ATCC 17978 WT; unpaired t test was performed: N.s. not significant; *P value ≤ 0.05; **P value ≤ 0.01; ***P value ≤ 0.001; ****P value ≤ 0.0001; ↑ more than ATCC 17978 WT; ↓ less than ATCC 17978 WT
eCompared to ATCC 17978 WT; unpaired t test was performed: N.s. not significant; *P value ≤ 0.05; **P value ≤ 0.01; ***P value ≤ 0.001; ****P value ≤ 0.0001; R resistant, S susceptible