. 2021 Mar 5;78(4):1509–1528. doi: 10.1007/s00284-021-02407-x

Table 3.

Minimal inhibitory concentration (MIC) of ampicillin, tetracycline and imipenem determined from ATCC 17978 wildtype/mutants and 29D2 wildtype/mutants

Locus tag	Gene name	Ampicillin^a		Imipenem^a		Tetracycline^a
Locus tag	Gene name	ATCC 17978	29D2	ATCC 17978	29D2	ATCC 17978	29D2
Wildtype		25.3	36.5	0.23	0.25	2.1	3.25
A1S_2187	purH	32	*18.6*	0.23	0.21	1.6	1.5
A1S_2251	purF	48	*16.0*	0.25	*0.16*	3.0	2.3
A1S_2605	purM	53.3	*17.3*	0.21	0.18	*1.0*	2.3
A1S_2964	purE	53.3	*10.6*	*0.13*	0.21	*1.5*	2.0
A1S_1566		96	*18.6*	0.23	0.23	1.8	*0.9*
A1S_2687	carB	32	*3.0*	*0.10*	*0.03*	*0.9*	2.0
A1S_0414		106.6	32.0	0.23	0.25	1.6	2.0
A1S_1624		32	–	*0.125*	–	2.1	–
A1S_0447	rpmG	29.3	–	*0.16*	–	*1.0*	–
A1S_0778	metG	32	–	0.23	–	*0.46*	–
A1S_0530		24	–	0.19	–	2.0	–
A1S_3366	gshA	85.3	*26.6*	0.29	0.23	1.6	2.0
A1S_1970		64	32.0	*0.16*	0.25	2.5	1.8
A1S_2840	ompA	29.3	*13.3*	0.46	*0.13*	*1.5*	3.0
A1S_3297		58.6	–	0.33	–	2.0	–
A1S_2453	ddc	32	*5.0*	0.46	*0.14*	*0.38*	4.6
A1S_2454	dat	26.6	*5.3*	0.25	0.23	*0.29*	6.0
A1S_0113		42.6	–	0.25	–	2.0	–
A1S_0116		85.3	29.3	0.21	0.33	2.6	1.5
A1S_0222	aamA	18.6	42.6	*0.125*	0.38	*0.9*	2.6
A1S_0065	galE	53.3	*13.3*	0.23	0.18	3.0	2.0
A1S_0806		53.3	*13.3*	0.38	*0.023*	2.0	1.6
A1S_1055		26.6	21.3	0.29	*0.14*	*1.3*	1.6
A1S_2182	gidA	37.3	*24.0*	0.19	0.25	3.0	4.0
A1S_2334	sahH	74.6	–	0.18	–	1.8	–
A1S_2587	ruvA	53.3	–	0.19	–	*1.0*	–
A1S_2610	comEC	29.3	*5.3*	0.23	*0.10*	1.6	*0.6*
A1S_2761	prpF	42.6	*14.6*	0.25	*0.16*	*0.8*	*0.5*
A1S_3026		106.6	64.0	0.42	0.46	2.6	1.5
A1S_3129	astB	74.6	–	0.23	–	*1.1*	–

A bold indicates that MIC values of mutant strains are significantly resistant compared to the wildtype and a bold italic indicates susceptibility

^aAveraged MIC values in (µg/mL) determined from three independent experiments. ‘–’ indicates ‘not tested’