Fig. 5.

Plasma from COVID-19 patients reduces the expression of glycocalyx components. Immunoblot representative images (A, B, C, and D) and densitometric analysis (right) of protein levels of syndecan-1, glypican-1, and syndecan-4 in HUVECs non-treated (Control), treated with plasma from healthy subjects and COVID-19 patients for 30 min (A), 1 h (B), 12 h (C) and 24 h (D). The values are normalized by β-actin and expressed by absolute values. Data are representative of at least 3 experiments and are shown as mean ± SEM. Student's t-test. *p < 0.05. We processed images of blots changing brightness and contrast and we applied equally over the entire image.