Fig. 6.

Heparin/LMWH abrogates the reduced levels of glycocalyx components induced by plasma of COVID-19 patients. Immunoblot representative images (up) and densitometry analysis (A, B, and C) of protein levels of syndecan-1, glypican-1, and syndecan-4 in HUVECs treated with plasma from healthy subjects and COVID-19 patients for 12 h in presence of Heparin/LMWH (10 μg/ml, 12 h). The values are normalized by β-actin and expressed as absolute values. Data are representative of at least 6 experiments and are shown as mean ± SEM. One-way ANOVA followed by Tukey post-test. *p < 0.05. We processed images of blots changing brightness and contrast and we applied equally over the entire image.