Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 26;12:1929. doi: 10.1038/s41467-021-22117-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a 2D differentiation into mature differentiated neurons (DNs). We analyzed DNs at 4 weeks (4w) and 8 weeks (8w) starting from NPCs. b HCA quantification of TUJ1 + neurons in 4w and 8w DNs from CTL (CTL_NoMut: C1, C2, C3; SURF1_NoMut: S2_Corr1, S2_Corr2) and SURF1 (SURF1_Mut: S1, S2; CTL_Mut: C1_Mut1) (mean ± s.e.m.; each dot represents a biological replicate; n = 20 biological replicates per line over three independent experiments; **p = 0.0013, ****p < 0.0001 CTL vs. SURF1; two-sided Mann–Whitney U test). c, d Bioenergetics of 4w DNs and 8w DNs from CTL (CTL_NoMut: C1, C2, C3; SURF1_NoMut: S2_Corr1, S2_Corr2) and SURF1 (SURF1_Mut: S1, S2; CTL_Mut: C1_Mut1) (mean ± s.e.m.; each dot represents a biological replicate; n = 20 biological replicates per line over three independent experiments; ****p < 0.0001 CTL vs. SURF1; two-sided Mann–Whitney U test). e, f Sodium (below x axis) and potassium (above x axis) currents in 4w and 8w DNs from CTL (CTL_NoMut: C1, C2, C3; SURF1_NoMut: S2_Corr1, S2_Corr2) and SURF1 (SURF1_Mut: S1, S2; CTL_Mut: C1_Mut1) (mean ± s.d.; n = 40 individual cells per line over three independent experiments; ***p < 0.001, ****p < 0.0001 CTL vs. SURF1; one-way ANOVA followed by Bonferroni multiple comparison test). g Electrophysiology traces in current-clamp recordings for 8w DNs from CTL (SURF1_NoMut: S2_Corr1) and SURF1 (SURF1_Mut: S2). Above: spiking activity; below: spontaneous postsynaptic activity; stars: glutamatergic postsynaptic currents. h 3D differentiation into cerebral organoids. We counted days of differentiation starting from embryoid bodies (EBs). i Cerebral organoids from CTL (SURF1_NoMut: S2_Corr1) and SURF1 (SURF1_Mut: S2) at D40 and D90. Reproduced in CTL (CTL_NoMut: C1, C2) and SURF1 (SURF1_Mut: S1; CTL_Mut: C1_Mut1) (3–8 organoids per line per experiment, n = 3 independent experiments). Scale bars: 100 µm.