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. 2021 Mar 26;12(4):326. doi: 10.1038/s41419-021-03494-1

Fig. 1. SMO facilitated HCC cell proliferation, migration, invasion, EMT process, and stemness characteristic.

Fig. 1

A qRT-PCR revealed the expression level of SMO in HCC cell lines (Hep 3B, LM3, 97H, and Huh-7) and HSC cell line (LX2). B qRT-PCR verified the depletion efficiency of SMO in LM3 and 97H cells. C–D EdU (bar value = 100 μm) and colony formation assay revealed in the proliferation of HCC cells under the depletion of SMO or not. E Wound-healing assay showed the migration ability of HCC cells with or without downregulated SMO (bar value = 80 μm). F–G Transwell assay revealed HCC cell migration and invasion ability in response to SMO silence (bar value = 50 μm). H Western blot detected the expression of E-cadherin, N-cadherin, OCT4, and Nanog in HCC cells transfected with sh-NC or sh-SMO#1/2. I Sphere formation assay revealed the sphere formation efficiency of HCC cells after silencing SMO (bar value = 50 μm). *P < 0.05, **P < 0.01.