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. 2021 Mar 26;12(4):326. doi: 10.1038/s41419-021-03494-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A RNA pull-down assay examined the enrichment of miR-326 and miR-330-5p by biotin-labeled SMO. B qRT-PCR examined the overexpression efficiency of miR-330-5p in activated HSCs. C qRT-PCR revealed the influence of upregulated miR-330-5p on SMO expression in activated HSCs. D Binding sites between SMO and miR-330-5p were predicted by starBase database. E Luciferase reporter assay examined the changes in luciferase activity of wild-type and mutant SMO in activated HSCs after overexpressing miR-330-5p. F RNA pull-down assay revealed the enrichment of four potential lncRNAs by biotin-labeled miR-330-5p. G qRT-PCR determined MIRLET7BHG expression in quiescent and activated HSCs. H qRT-PCR validated the depletion efficiency of MIRLET7BHG and also evaluated the influence of silenced MIRLET7BHG on SMO expression in activated HSCs. I LncLocator database and subcellular fraction assay revealed the subcellular location of MIRLET7BHG in activated HSCs. J Binding sites between MIRLET7BHG and miR-330-5p were predicted by starBase database. K RNA pull-down assay revealed the enrichment of MIRLET7BHG pulled down by biotin-labeled miR-330-5p. L RIP assay analyzed the enrichment of MIRLET7BHG, miR-330-5p, and SMO in anti-Ago2 group relative to anti-IgG group. M Luciferase reporter assay detected the changes in luciferase activity of wild-type and mutant MIRLET7BHG under miR-330-5p overexpression. **P < 0.01, “n.s” represents no significance.