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. 2021 Mar 26;12(4):326. doi: 10.1038/s41419-021-03494-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A qRT-PCR revealed the influence of silenced SMO on MIRLET7BHG expression. B Luciferase reporter assay disclosed the luciferase activity of MIRLET7BHG promoter when SMO was subjected to downregulation. C Western blot tested hedgehog pathway-associated proteins (SMO and Gli1) in indicated groups. D qRT-PCR verified the overexpression efficiency of Gli1 and the influence of upregulated Gli1 on MIRLET7BHG expression. E qRT-PCR tested the knockdown efficiency of Gli1 and the influence of downregulated Gli1 on MIRLET7BHG expression. F Luciferase reporter assay analyzed the luciferase activity of MIRLET7BHG promoter in activated HSCs with or without the upregulation of Gli1. G DNA motif of Gli1 and two binding sites of Gli1 on MIRLET7BHG promoter. H ChIP assay revealed the enrichment of MIRLET7BHG promoter in anti-Gli1 group relative to control anti-IgG group. I Luciferase reporter assay determined the impact of Gli1 overexpression on the luciferase activity of reporters with indicated MIRLET7BHG promoter (wild-type, site 1-mutated type, site two-mutated type, or site 1/2-mutated type). *P < 0.05, **P < 0.01, “n.s” represents no significance.