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. 2021 Mar 26;12(4):325. doi: 10.1038/s41419-021-03618-7

Fig. 6. Increased TEFM expression is mainly mediated by down-regulation of miR-194-5p.

Fig. 6

A TEFM expression was analyzed by qRT-PCR in HUH-7 and HLF cells transfected with synthetic precursors of miR-194-5p, miR-216-5p, miR-376a-3p, and miR-376b-3p. B TEFM expression were analyzed by western blot in HUH-7 and HLF cells transfected with synthetic miR-194-5p precursor. C Correlation between TEFM and miR-194-5p in tumor tissues of HCC (n = 30). D TEFM expression were analyzed by western blot in HUH-7 and HLF cells transfected with TEFM expression vector and synthetic miR-194-5p precursor. E, F Cell proliferation was analyzed by MTS and colony formation assays in HUH-7 and HLF cells transfected with TEFM expression vector and synthetic miR-194-5p precursor. G, H Cell metastasis was analyzed by wound-healing and transwell Matrigel invasion assays in HUH-7 and HLF cells transfected with TEFM expression vector and synthetic miR-194-5p precursor.