Fig. 1. USP15 knockdown suppresses the growth of ERα⁺ BC cells.

A, B T47D and MCF-7 cells were treated with USP15 siRNA for 24, 48, and 72 h. C MCF-7 cells stably expressing USP15 shRNA were cultured in 96-wells plates for 4 and 6 days. 20 μl MTS was applied to react for this test of cell viability. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 versus each vehicle control. D Cells were transfected with USP15 siRNA or MCF-7 cells were transfected with different sets of USP15 siRNA for 48 h. Edu agent was added and subjected to fluorescence microscope. E Stained cells by Edu were counted. ^*p < 0.05, ^**p < 0.01 versus each vehicle control. f Colony formation assay was carried out in MCF-7 treated with USP15 shRNA for 10-14 days.