Fig. 2. USP15 deletion triggers cell cycle arrest in ERα+ BC cells.
A, C Cells were exposed to USP15 siRNA targeting different sets for 48 h, subjected to FACS for cell distributions. B, D The percent of cell number on each phase was showed. E, F Cells were treated with USP15 siRNA, proteins were collected for USP15, CDK4, Cyclin D1, Rb, p-Rb, p21, and p27 expression. GAPDH was as a normalizer. G The same treated cells were tested using flow cytometry for apoptotic cells and H the pooled data has been shown. NS is no significant.