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. 2021 Mar 26;12(4):329. doi: 10.1038/s41419-021-03607-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Proteins were extracted from breast cancer cells. Immunoprecipitated with ERα or USP15, immunoblot with USP15 or ERα. C Proteins from HEK293T cells treated with FLAG-tagged ERα and MYC-tagged USP15. Co-IP and western blot assay were applied for MYC and FLAG. D MCF-7 and T47D cells were treated with FLAG-tagged ERα and then incubated with anti-USP15 and anti-FLAG antibodies. Images were captured by fluorescence microscope.