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. 2021 Mar 26;12:1901. doi: 10.1038/s41467-021-22267-0

Fig. 4. AP-1, but not AP-4, buds from the TGN.

Fig. 4

a–d 3D time-lapse (4D) images of iRFP-SYP61, AP1M2-mRFP, and AP4M-GFP in the epidermal cells of the root elongation zone under SCLIM. Arrowheads indicate the dissociation of AP1M2-mRFP and iRFP-SYP61 from a large population of TGN labeled with iRFP-SYP61. Images are lined up every 6.4 s (a) or 3.1 s (b–d) from left to right. Scale bars = 1 μm. e–h Time course changes in relative fluorescence intensities of iRFP-SYP61, AP1M2-mRFP, and AP4M-GFP in ROIs of a–d. The experiments were repeated independently six times with similar results, and micrographs from representative experiments are presented.