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. 2021 Mar 26;12:1901. doi: 10.1038/s41467-021-22267-0

Fig. 6. Temporal relation between and ultrastructure of the Golgi apparatus, TGN, and secretory trafficking zone component clathrin.

Fig. 6

a 4D images of ST-iRFP, GFP-SYP61, and CLC2-mKO in the epidermal cell of the root elongation zone under SCLIM. White arrowheads indicate dissociation of CLC2 and SYP61 subpopulation from the major population of the TGN (GA-TGN) labeled with GFP-SYP61. b Magnified images of boxed area in a. Images are lined up every 6.4 s from left to right. c Time course changes in relative fluorescence intensities of ST-iRFP, GFP-SYP61, and CLC2-mKO in the Golgi/GA-TGN or GI-TGN area in a. d, e Transmission electron microscopic images of Arabidopsis root epidermal cells. Arrowheads indicate clathrin-coated vesicles/buds. Boxed area shows the CCV cluster. The experiments were repeated independently at least three times with similar results, and micrographs from representative experiments are presented. Scale bars = 1 μm (a); 500 nm (b); 200 nm (d, e).