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. 2021 Mar 26;12:1924. doi: 10.1038/s41467-021-22145-9

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a HeLa cells transfected with PINK1 siRNA were co-transfected with empty vector (EV) or FLAG-tagged CHCHD10^S59L and FLAG-tagged TARDBP^WT. Fractionated mitochondria were analyzed with anti-TDP-43 (arrowhead indicates transfected TDP-43) or anti-HSP60 and anti-Cox2 (loading controls) antibodies. Data are shown as mean ± SD (two-sided t test, NS = not significant; n = 4 independent experiments). b HeLa cells transfected with PINK1 siRNA were co-transfected with empty vector (EV) or FLAG-tagged CHCHD10^S59L and TARDBP^A382T. Fractionated mitochondria were analyzed with anti-TDP-43 (arrowhead indicates transfected TDP-43) or anti-HSP60 and anti-Cox2 (loading controls) antibodies. c HeLa cells transfected with PINK1 siRNA were transfected with FLAG-tagged CHCHD10^S59L. RIPA-soluble and insoluble fractions were analyzed with anti-FLAG and anti-actin (loading control) antibodies. Data are shown as mean ± SD (two-sided t test, NS = not significant; n = 3 independent experiments). d HeLa cells transfected with PINK1 siRNA were co-transfected with FLAG-tagged CHCHD10^S59L and TARDBP. RIPA-soluble and insoluble fractions were analyzed with anti-TDP-43 and anti-actin (loading control) antibodies. Data are shown as mean ± SD (two-sided t test, NS = not significant; n = 4 independent experiments). e HeLa cells transfected with PINK1-YFP, FLAG-tagged CHCHD10^S59L, and HA-tagged CHCHD10^WT or EV were visualized with anti-FLAG (blue) and anti-HA (red) antibodies and YFP (yellow) after 24 h of transfection. PINK1-positive cells with/without CHCHD10^WT in CHCHD10^S59L-expressing cells were counted (two-sided t test, **p = 0.00335; n = 3 independent experiments, >50 cells for each group). Scale bar = 20 μm. f HeLa^PINK1-V5-His cells transfected with EV or CHCHD10^WT were treated with CCCP (10 μM) for 6 h. Cells were immunostained with anti-FLAG (green) and anti-V5 (red) antibodies to visualize CHCHD10 and PINK1, respectively. Arrow indicates PINK1 accumulated in a non-transfected cell neighboring a CHCHD10-transfected cell (white dashed line). The percentage of PINK1-positive cells from the empty vector (EV) or CHCHD10^WT-transfected cells were calculated after 5 or 10 μM CCCP treatment for 6 h. Data are shown as mean ± SD (two-sided t test, **p = 0.009055 for 5 μM and 0.009203 for 10 μM CCCP treatment; n = 3 independent experiments, >200 cells for each group). Scale bar = 20 μm.