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. 2021 Mar 26;4:417. doi: 10.1038/s42003-021-01941-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Scheme of colony formation assays (CFAs) with relative CENP-A protein levels over time for TetOn-CENPA cell lines. b CFA results corresponding to the scheme in (a) for five different cell lines of varied tissue origins and p53 status, as indicated. Each dot represents a single biological replicate. Plots show mean and 95% confidence interval. Statistical significance tested by two-tailed Welch’s t test with Bonferroni cutoff at a p value of 0.01 (α = 0.05). *** = p value < 0.0001. See also Supplementary Fig. 1C for survival ratios relative to untreated condition. c Western blot corresponding to scheme in (a): TCEs after 1 day (1d) or 4d with 10 ng/ml Dox, no Dox control (0d), or 4d Dox followed by 4d without Dox (Rev). Primary antibodies indicated on the left. 1x load = ~30,000 cells. # = high sensitivity ECL exposure. y-tubulin used as loading control. d CFA results corresponding to scheme in (a) for the indicated cell lines. Plots as in (b). Statistical significance tested by two-tailed Welch’s t test, compared to non-induced control, with Bonferroni cutoff at a p value of 0.0125 (α = 0.05). *** = p value < 0.0001.