Table 1.
Description of cell lines used and their p53 status.
| Cell line | p53 statusa | Cell type | Construct | Comments |
|---|---|---|---|---|
| MCF10-2A | Functional wildtype | Immortal non-tumoral (fibrocystic disease) breast epithelial | TetOn-CENP-A-FLAG-HA (lentiviral) | Female origin |
| HCT116 | Functional wildtype | Colon cancer | TetOn-CENP-A-FLAG-HA (lentiviral) | Male origin |
| HeLa S3 | Nonfunctional wildtype: HPV18 inactivated | Cervical cancer | TetOn-CENP-A-FLAG-HA (lentiviral) | Female origin |
| HCC1954 | LOF p53 mutation at Y163C | Invasive ductal carcinoma, breast | TetOn-CENP-A-FLAG-HA (lentiviral) | Female origin; HER+ ER− PR− luminal |
| DLD1 | One functionally silent allele of p53 and LOF mutation at S241F | Colon cancer | TetOn-CENPA-YFP-AID (FlpIn) | Male origin; kind gift from D. Fachinetti |
ap53 status for missense mutations from the TP53 database107.